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KMID : 0613820150250090993
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Journal of Life Science
2015 Volume.25 No. 9 p.993 ~ p.999
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Inhibition of Inflammation by Popillia flavosellata Ethanol Extract in LPS- induced RAW264.7 Macrophages
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Yoon Young-Il
Hwang In-Yeong
Kim Mi-Ae
Ahn Mi-Young
Lee Young-Bo
Han Myung-Se
Goo Tae-Won
Yun Eun-Young
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Abstract
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The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.
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KEYWORD
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Anti-inflammation, lipopolysaccharide, Popillia flavosellata, pro-inflammatory cytokine, RAW 264.7 cells
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